Although DNA microarray technologies are very powerful for the simultaneous quantitative characterization of thousands of genes, the quality of the obtained experimental data is often far from ideal. The measured microarrays images represent a regular collection of spots, and the intensity of light at each spot is proportional to the...
Whole genome amplification WGA promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA gDNA quantity. The paper evaluates the performance of multiple displacement amplification MDA WGA using gDNA extracted from lymphoblastoid cell lines (N=27) with a range of starting gDNA input of 1-200 ng into the WGA...
The paper deals with development of an oligonucleotide micro array genosensor utilizing a double tandem hybridization technique to search for 9 point mutations located in the most frequently altered codons of the TP53 gene. Isolated and multiplexed PCR products, 108 and 92 bp long, from exons 7 and 8, respectively,...
This paper introduces a novel and highly reliable single-tube method for rapid cell lysis, followed by quantitative preparation and analysis of both RNA and/or DNA molecules in small samples. In contrast to previous approaches, this procedure allows all steps to be carried out by sequential dilution in a single tube,...
Microarray data normalization is an important step for obtaining data that are reliable and usable for subsequent analysis. One of the most commonly utilized normalization techniques is the locally weighted scatterplot smoothing LOWESS algorithm. However, a much overlooked concern with the LOWESS normalization strategy deals with choosing the appropriate parameters....
This white paper document emphasizes on the fact that the complexity of parasitic infections requires novel approaches to vaccine design. The versatility of DNA vaccination provides new perspectives. This review discusses the use of prime-boost immunizations, genetic adjuvants, multivalent vaccines and codon optimization for optimal DNA vaccine design against parasites.
This white paper deals with Non-viral vectors for gene transfer are less immunogenic than viral vectors but also less efficient. Significant effort has focused on enhancing non-viral gene transfer efficiency by increasing nuclear import of plasmid DNA, particularly by coupling nuclear localization peptidic sequences to plasmid DNA. It reveals conclusion...
The conventional solution-phase Chemical Cleavage of Mismatch CCM method is time-consuming, as the protocol requires purification of DNA after each reaction step. This paper describes a new version of CCM to overcome this problem by immobilizing DNA on silica solid supports. It concludes that the new solid-phase version of CCM...
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