This white paper reports about a genetic system based on suicide vectors on conjugative transposons allowing stable integration of recombinant DNA into the chromosome of transformable and non-transformable Gram-positive bacteria. The aim of this work was to select a strong chromosomal promoter from Streptococcus gordonii to improve this genetic system...
A reference karyotype of tetraploid alfalfa (Medicago sativa subsp. sativa, 2n = 4x = 32) African Population germplasm source (PI 536539) was constructed using the combined techniques of C-banding and image analysis. The image analysis system is a metho A reference karyotype of tetraploid alfalfa (Medicago sativa subsp. sativa,...
Resistance genes for leaf rust (Puccinia recondita Rob. ex Desm.) and greenbug Schizaphis graminum Rondani were transferred from chromosome 7S of Triticum speltoides Tausch Gren. to chromosome 7A of hexaploid wheat (Triticum aestivum L.) by means of Resistance genes for leaf rust (Puccinia recondita Rob. ex Desm.) and greenbug...
Tobacco mosaic virus TMV-resistant flue-cured tobacco (Nicotiana tabacum L.) cultivars have been developed using the N gene derived from N. glutinosa L. Their adoption has been low, however, because of unfavorable linkage drag effects. Strategies to o Tobacco mosaic virus TMV-resistant flue-cured tobacco (Nicotiana tabacum L.) cultivars have been...
Because of their great importance as cytogenetic and breeding tools, haploids have been produced in several crop plants, including wheat (Triticum aestivum L.). Reports of seedset on haploid plants are very rare. Earlier, we produced 142 haploids (2n = Because of their great importance as cytogenetic and breeding...
Gray leaf spot GLS of maize (Zea mays L.) caused by Cercospora zeae-maydis Tehon & E.Y. Daniels, can greatly reduce grain yield in conducive environments worldwide. This study was undertaken to evaluate a novel source of resistance to C. zeae-mayd Gray leaf spot GLS of maize (Zea mays L.)...
The brown planthopper BPH, Nilaparvata lugens Stal (Homoptera: Delphacidae), is one of the major insect pests of rice (Oryza sativa L.). Many major genes are now available to manage this pest through host-plant resistance. In this study, we mapped q The brown planthopper BPH, Nilaparvata lugens Stal (Homoptera: Delphacidae),...
A non-Robertsonian wheat-rye chromosome translocation was observed in pollen-derived line WER-1-1 from the F1 hybrid between M27 [a 1R(1D) substitution line] and `Chinese Spring'. Biochemical assays of high molecular weight glutenin subunits and gliadin cA non-Robertsonian wheat-rye chromosome translocation was observed in pollen-derived line WER-1-1 from the F1 hybrid between...
Genetic analyses of complex traits in wheat (Triticum aestivum L.) are facilitated by the availability of unique genetic tools such as chromosome substitution lines and recombinant inbred chromosome lines RICLs which allow the effects of genes on sing Genetic analyses of complex traits in wheat (Triticum aestivum L.) are...
Rust resistance genes Lr37, Sr38, and Yr17 are located within a segment of Triticum ventricosum Tausch Cess. chromosome 2NS translocated to the short arm of bread wheat chromosome 2AS. Characterization of this chromosome segment by 13 restriction frag Rust resistance genes Lr37, Sr38, and Yr17 are located within a...
Chromosome 3A of bread wheat (Triticum aestivum L.) cultivar `Wichita' (W1) was previously found to differ from that of `Cheyenne' CNN for genes affecting a number of agronomically important traits such as grain yield GYLD, kernel number [spike.sup. Chromosome 3A of bread wheat (Triticum aestivum L.) cultivar `Wichita' (W1)...
Kernel color and grain protein concentration GPC are two of the most important attributes of barley (Hordeum vulgate L.) grain intended for use in malting and brewing. Grain with protein levels that are too low or high or that have dark kernel color, Kernel color and grain protein...
CAMBRIDGE, Mass. -- Helicos BioSciences Corporation (NASDAQ:HLCS) today announced a scientific collaboration with a respected genomics research group at Sweden's Uppsala University aimed at gaining new insights into how genome structure plays a key role in the ways genes are regulated within cells. The Uppsala team, led by...
RNAa May Have Applications in Many Human Diseases, Including Certain Genetic Disorders and Cancer Company Completes Exclusive License Agreements with University of Texas Southwestern Medical Center, University of California San Francisco, and the Salk Institute for Biological Studies CAMBRIDGE, Mass. -- Alnylam Pharmaceuticals, Inc. (Nasdaq: ALNY),...
When my nine-year-old daughter, Daisy, died, a doctor at the hospital said to me: "It's almost like losing a child." What did he think my beautiful daughter was? Daisy had gone into hospital with a tooth infection. Three weeks later she was dead. Her experience echoes many...
PARIS AFP — Three studies published on Wednesday unveil flaws in several chromosomes that highlight an inherited vulnerability to schizophrenia. Among people with schizophrenia, deletions and duplications are more common in key locations of the genetic code compared to otherwise healthy counterparts, according to the papers, released by...
MADISON, Wis. -- Chromosomal rearrangements leading to genomic disorders are often mediated by low-copy repeat regions of the genome (e.g. segmental duplications). Roche NimbleGen CGH arrays offer expanded probe coverage in these regions. Using this technique, the authors of a recently published paper1 identified a recurrent reciprocal genomic rearrangement of...
DENVER -- The Colorado Center for Reproductive Medicine CCRM announced today an increase in in vitro fertilization IVF success rates to 80 percent from its Institutional Review Board-approved clinical preimplantation genetic screening PGS study involving comprehensive screening of all 23 pairs of chromosomes on day five embryos blastocysts. ...
RICHMOND, Texas, July 30 /PRNewswire/ -- DNA Heritage has recently overcome patent claims held by a competitor which would have severely restricted the use of DNA testing and databases that allow families around the world to match and connect up through their DNA. The patent covers the use of...
BEIJING AFP — Olympic host Beijing has set up a sex determination lab to test female Olympic athletes suspected to be males, state media reported Sunday. Experts at the lab, located at the Peking Union Medical College Hospital, will evaluate dubious cases based on their external appearance and...